Genymotion virtual devices are all rooted and it is not possible to unroot them for the moment.
However, we will implement non-rooted devices in the future. To help us in this task, feel free to fill our feature request form: https://forms.gle/1jsegyt4xr33Mt3z6.
Note
In some cases, it is possible to hide root from an application. To do so, please refer to:
How to use Xposed/Edxposed to hide root access from an application? (Genymotion Desktop)
How to use Xposed/Edxposed to hide root access from an application with Genymotion SaaS?
How to use Xposed/Edxposed to hide root access from an application in Genymotion Device image (PaaS)?